Jan 03, 2018

A team from the University of British Columbia and the University of California, Santa Cruz, presents information on genome sequences generated for the model organism Caenorhabditis elegans using the MinION nanopore long read sequencer. With MinION sequencing and de novo assembly, the researchers tackled the genomes of wild type and rearrangement-containing C. elegans strains. In the wild-type strain, they report, the resulting assembly spanned more than 99 percent of the existing reference genome, while stretching it out by more than two million bases and highlighting sequences for co-occurring bacteria. Along with analyses of complex rearrangements revealed in the MinION-sequenced mutant C. elegans genome, the authors improved the accuracy of the wild type assembly by incorporating Illumina short reads.

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