April 11, 2018

University of California, Santa Cruz, researchers present a single-cell RNA sequencing method called Tn5Prime — a Smart-seq2 protocol that includes a Tn5 transposase-based 5′-capture step. “The Tn5Prime method dramatically streamlines the 5′ capture process and is both cost effective and reliable,” according to investigators, who applied the approach to both bulk RNA and individual cells — primary mouse B lymphocytes or human immune B cells isolated by peripheral blood mononuclear cell sorting — to generate quantifiable transcriptome data that included transcription start sites as well as assemblies for antibody light and heavy chains.

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